Part:BBa_C0076

autoinducer synthetase This part codes for an autoinducer synthetase which utilizes SAM to make the quorum sensing molecule 3OH,C14:1-HSL.
3OH,C14:1-HSL can bind CinR (Part:BBa_C0077) to make a complex which can activate the Cin promoter Part:BBa_R0077.

Usage and Biology

In Rhizobium leguminosarum, cinR and cinI are forward-pointing, adjacent, and separate transcriptional elements. There is a short (<200bp) intergenic region between cinR and cinI. The cinR/intergenic/cinI structure is conserved for the cinRI locus in Rhizobium etli, and for the cerRI locus in Rhodobacter sphaeroides. The intergenic region is thought to contain a terminator for cinR, a promoter and RBS for cinI, and a dna-binding domain for cinR and its homologs, though a conserved structure has not yet been identified.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 223
  
• 1000
  COMPATIBLE WITH RFC[1000]